Application

ELISA: 0.5 µg/mL this dilution will allow detection (in direct ELISA) of

0.2-0.4 ng/well when rat TNF-α is used to coat the plate.

Western blot: 0.1 - 0.2 µg/mL, this dilution should offer detection limit of 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions.

Optimal working dilutions must be determined by the end user.

Research CategorySignaling

Research Sub CategoryGrowth Factors & Receptors

Anti-Tumor Necrosis Factor-α Antibody is an antibody against Tumor Necrosis Factor-α for use in ELISA & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Highly purified (>98%) recombinant rat TNF-alpha

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1114

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified Immunoglobulin. Lyophilized from PBS pH 7.2. Contains no preservative. Reconstitute with sterile distilled water.

Specificity

Reacts with rat TNF-alpha

Storage and Stability

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile water to a concentration of =0.2 mg/ml the antibody is stable for at least six weeks at 2-4°C.